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2nd Edition of International Cancer Research Conference

March 19-21, 2026 | Singapore

March 19 -21, 2026 | Singapore
Cancer Research 2025

Efficient anticancer properties of ashwagandha extracted phytochemicals for lower protein expression on U-251 and clove extracts on LN-229 GBM cell lines

Speaker at International Cancer Research Conference 2025 - Prabha Muddobalaiah
Ramaiah Institute of Technology, India
Title : Efficient anticancer properties of ashwagandha extracted phytochemicals for lower protein expression on U-251 and clove extracts on LN-229 GBM cell lines

Abstract:

Glioblastoma Multiforme G IV primary malignant Brain tumor medications such as Radiation, Surgery, Gene therapy, Chemotherapy and Ayurveda which are not very much successful with clinical studies. However, the trials are being made towards the various cancers’ successful treatment with less side effects. Hence natural phytochemicals extract of Ashwagandha (Withania somnifera) Soya bean (Glycine max) and Clove (Syzygium aromaticum) are also considered for cancer therapeutics having much side effects on Human brain cells and organs.

The Phytochemicals treated GBM cell lines U251 and LN229 subjected for MTT assay, SDS gel electrophoresis, Protein content determination by  Lowry and Bradford assays.

The Ashwagandha extracted phytochemicals such as Alkaloids, tannins, saponins, phlobatannins, Flavonoids, Terpenoids were determined. Ashwagandha phytochemicals extract treated U251 cells showed IC50-1.1 µg/100 µl and 83.95% cell death for the same concentration.

The LN 229 Cell viability reduced to 40% with clove phytochemicals and cinnamaldehyde.

The protein content reduced to 118.68µg/ml for 138µl/ml Ashwagandha phytochemicals as compared to control protein 257.25 µg/ml. Whereas, less protein band intensity displayed for phytochemicals of Ashwagandha, treated U251 cells and less protein expressed for cinnamaldehyde and clove extract phytochemicals treated LN229 cells respectively when compared to  their control.

Therefore, GBM U251 and LN 229 cells treated with Phytochemicals of Ashwagandha and clove showed reduction in protein expression directly depicts tumor cell death that acts as anticancer drug conjugate.

Key words: GBM Cells; U 251; LN229; Phytochemicals; Protein expression; Tumor cell death.

Biography:

Dr. Prabha M is an Associate Professor at RIT, located in Bengaluru, India. She has published 20 research articles and 8 conference proceedings publications. She was Post doctoral Fellow for CSIR RA fellowship 2008 at IISc. She has received the Distinguished woman in health and medical sciences-Biochemistry — From Venus International foundation, Chennai. 2019. She has been awarded as Bharat Shiksha Gaurava Puraskar – 2022 and Excellence for Best Educationist Award-2022 by KTK foundation, New Delhi. She got certificate for NPTEL Domain star–Biotechnology—Biosciences 2022. She has received the certificate for winner for Women Researcher Award– In the International Scientist Awards on Engineering, Science and Medicine, held on 04-Nov-2022, Organized by VDGOOD® Professional Association. She is an invited editor for Journal of Clinical Science & Translational Medicine, Journal of Biomedicine and Biosensor and Biochemical Engineering & Bioprocess Technology. She is invited as reviewer constantly for. Clinical and Translational oncology Springer publications, Cancer Biomarker, Journal of Neurochemistry and for Indian journal of Neurosciences. She is guiding PhD and many MTech /BE project students.

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