Abstract
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Background: Multiforme (GBM) are tumours arising from astrocytes, which are transformed, and the supportive cells of neurons. These tumours are highly malignant due to high proliferation capacity, angiogenesis, activation of oncogenes, high expression of cancer proteins and enzymes such as kinases.
Purpose: Therefore, it is essential to study the effect of inhibition of various proteins, enzymes and their expression levels in cell lines. β-galactosidase hydrolytic enzymes when expressed in abnormal levels have major impact in cancer progression. Higher expression of β-galactosidase has been reported in glioblastoma tissue, which promotes the degradation of glycol-conjugates present in the ECM and promotes tumor infiltration and metastasis.
Method: It was aimed to study the effect of chemical modulator (Lithium) and indigenous modulator (Curcuma longa extract) on β-galactosidase in LN229 cell lines.
Results: Total protein content (Lowry’s assay) significantly reduced with an increase in concentration of both the modulators (Li-1µM,2µM,5µM,10µM), Curcuminoid-2.5ppm,5ppm,10ppm,25ppm) in cell line and spent media. β-Galactosidase content and specific activity in cells were found to be downregulated in cells and upregulated in spent media.
Conclusion: Hence, it was concluded that both the modulators Lithium and Turmeric extract were negative for β-galactosidase dealing with lysosomal activity and failure of membrane permeability. These results pave way for its use as conjugates with anti-cancer drugs.
Key words: Glioblastoma Multiforme (GBM) cell lines LN229; Acid Phosphatase; β-Galactosidase; Lithium; Turmeric; Spent media
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